Timeplan 2017 Høst - lærer Kenneth Bowitz Larsen - Overingeniør
Kenneth Bowitz Larsen
Uke 36
Mandag
08:15-10:00 - 04.09.2017
Lecture
FOR: Lecture: Introduction to MBI-3010 and presentation of lecturers
FOR: Lecture: Introduction to MBI-3010 and presentation of lecturers
Tirsdag
Onsdag
Torsdag
Fredag
Uke 45
Mandag
08:15-10:00 - 06.11.2017
Lecture
FOR: Lecture: Introduction. Cultured cells as a model system in basic research. Mammalian cell culture. Gene delivery for fluorescence microscopy.
FOR: Lecture: Introduction. Cultured cells as a model system in basic research. Mammalian cell culture. Gene delivery for fluorescence microscopy.
11:15-12:00 - 06.11.2017
Lab work
LAB: Lab work: HeLa cells - characteristics and morphology. NB! L9.142
LAB: Lab work: HeLa cells - characteristics and morphology. NB! L9.142
13:15-15:00 - 06.11.2017
Lab work
LAB: Labwork: Mammalian cell culture. trypsinize, count, sub-culture; seed cells for immunofluorescence. Reverse transfection.
LAB: Labwork: Mammalian cell culture. trypsinize, count, sub-culture; seed cells for immunofluorescence. Reverse transfection.
15:15-16:00 - 06.11.2017
Lecture
FOR: Demo: Confocal microscopy basics. NB! Room L8.146
FOR: Demo: Confocal microscopy basics. NB! Room L8.146
Tirsdag
09:15-10:00 - 07.11.2017
Lab work
LAB: Lab work: Inhibitor treatments
LAB: Lab work: Inhibitor treatments
10:15-12:00 - 07.11.2017
Lecture
FOR: Lecture: Lab report/discussion. Confocal microscopy. applications of confocal microscopy. Immunofluorescence.
FOR: Lecture: Lab report/discussion. Confocal microscopy. applications of confocal microscopy. Immunofluorescence.
13:15-16:00 - 07.11.2017
Lab work
LAB: Lab work: Immunofluorescence. Fix transfected cells
LAB: Lab work: Immunofluorescence. Fix transfected cells
Onsdag
09:15-12:00 - 08.11.2017
Lab work
LAB: Lab work: Confocal microscopy (immunofluorescence) NB! Room L8.142/6
LAB: Lab work: Confocal microscopy (immunofluorescence) NB! Room L8.142/6
13:15-16:00 - 08.11.2017
Lab work
LAB: Lab work: Confocal microscopy (transfection). NB! Room L8.142/6
LAB: Lab work: Confocal microscopy (transfection). NB! Room L8.142/6
Torsdag
13:15-16:00 - 09.11.2017
Lab work
LAB: Lab work: Image analysis using Volocity. NB! Room L8.142
LAB: Lab work: Image analysis using Volocity. NB! Room L8.142
Fredag
09:15-10:15 - 10.11.2017
Lecture
FOR: Lecture: Labreport/discussion
FOR: Lecture: Labreport/discussion
10:15-12:00 - 10.11.2017
Lab work
LAB: Lab work: Confocal microscopy. Image analysis. Lab report. NB! Room L8.142/6
LAB: Lab work: Confocal microscopy. Image analysis. Lab report. NB! Room L8.142/6
13:15-14:00 - 10.11.2017
Lab work
LAB: Lab work: Seed FlpIn cells for flow cytometry (groups 1&2) and live cell imaging (group 3&4
LAB: Lab work: Seed FlpIn cells for flow cytometry (groups 1&2) and live cell imaging (group 3&4
14:15-16:00 - 10.11.2017
Lab work
LAB: Lab work: Confocal microscopy. Image analysis. Lab report. NB! Room L8.142/6
LAB: Lab work: Confocal microscopy. Image analysis. Lab report. NB! Room L8.142/6
Uke 46
Mandag
08:15-09:00 - 13.11.2017
Lab work
LAB: Lab work: Induce GFP-p62 expression in FlpIn cells
LAB: Lab work: Induce GFP-p62 expression in FlpIn cells
09:15-12:00 - 13.11.2017
Lecture
FOR: Lecture: Introduction to flow cytometry.
FOR: Lecture: Introduction to flow cytometry.
Tirsdag
08:15-09:00 - 14.11.2017
Lab work
LAB: Lab work: Start washout series for flow cytometry (6h washout). Start BioStation (control medium)
LAB: Lab work: Start washout series for flow cytometry (6h washout). Start BioStation (control medium)
09:15-10:00 - 14.11.2017
Lecture
FOR: Lecture: Fundamentals of digital imaging.
FOR: Lecture: Fundamentals of digital imaging.
10:15-12:00 - 14.11.2017
Lab work
LAB: Workshop: Creating figures in photoshop
LAB: Workshop: Creating figures in photoshop
13:15-14:00 - 14.11.2017
Lab work
LAB: Lab work: Continue working with figures in photoshop. lab7discussion
LAB: Lab work: Continue working with figures in photoshop. lab7discussion
14:15-16:00 - 14.11.2017
Lab work
LAB: Labwork: Flow cytometry using FlpIn cells. Restart BioStation (starvation medium)
LAB: Labwork: Flow cytometry using FlpIn cells. Restart BioStation (starvation medium)
Onsdag
09:15-10:00 - 15.11.2017
Lecture
FOR: Lecture: Analysis "how to" BioStation and flow cytometry data.
FOR: Lecture: Analysis "how to" BioStation and flow cytometry data.
10:15-12:00 - 15.11.2017
Lab work
LAB: Labwork: Analysis BioStation and flow cytometry data. NB! room L8.142
LAB: Labwork: Analysis BioStation and flow cytometry data. NB! room L8.142
13:15-14:00 - 15.11.2017
Lab work
LAB: Lab work:Split and count HeLa cells. Revers Transfection for cell sorting.
LAB: Lab work:Split and count HeLa cells. Revers Transfection for cell sorting.
14:15-16:00 - 15.11.2017
Lab work
LAB: Lab work: Confocal microscopy. Data analysis. Lab report. NB! Room L8.142/6
LAB: Lab work: Confocal microscopy. Data analysis. Lab report. NB! Room L8.142/6
Torsdag
09:15-10:00 - 16.11.2017
Lecture
FOR: Lecture: Advanced methods in confocal microscopy.
FOR: Lecture: Advanced methods in confocal microscopy.
10:15-12:00 - 16.11.2017
Lab work
LAB: Demo: Functional imaging usin FRAP. NB! Room L8.146
LAB: Demo: Functional imaging usin FRAP. NB! Room L8.146
13:15-16:00 - 16.11.2017
Lecture
FOR: Lecture: Labreport. Final discussions
FOR: Lecture: Labreport. Final discussions
Fredag
09:15-10:00 - 17.11.2017
Lab work
LAB: Labwork: Fix sorted cells
LAB: Labwork: Fix sorted cells
10:15-12:00 - 17.11.2017
Lab work
LAB: Labwork: Confocal microscopy of sorted cells. NB! L8.142/6
LAB: Labwork: Confocal microscopy of sorted cells. NB! L8.142/6
Uke 48
Mandag
12:15-16:00 - 27.11.2017
Lab work
LAB: Labwork: TEM practice (groups) aterning with demonstration of cryoultramicrotomy (groups) L7. EM department
LAB: Labwork: TEM practice (groups) aterning with demonstration of cryoultramicrotomy (groups) L7. EM department
Tirsdag
Onsdag
Torsdag
Fredag